Characterization & Validation of Research Antibodies

Adding More Value to Research Antibodies
What can make research antibodies more valuable to customers? Validated isotype, defined species and verified application data! Customers want to see application specific data for antibodies. Human tissues are becoming more widely used in drug target validation by immunohistochemistry, but validated human data for antibody validation is difficult to find. So why not rely on the leader in human tissue-based research and the leading global supplier of human tissues and biofluids for research purposes?

Asterand combines their renowned human tissue-based^® IHC services with their TargetEvaluator^® Database containing ~2000 XpressWay^® Profiles to provide real tangible value for research antibodies.

Left: Our PhaseZERO® team views XpressWay® Profile data to identify and/or confirm the tissue types in which your target shows optimal expression for imaging representation. Center: Optimal IHC protocols are confirmed and performed, whereby Right: high quality digital photomicrographs are produced and provided for your marketing efforts.

Increased antibody value and marketability is as easy as 1-2-3
Step 1:
Asterand's PhazeZERO^® Target Validation Service group will look for a suitable positive control tissue by mining the TargetEvaluator^® Database which holds gene expression data for over 2000 druggable targets across 72 human tissue types each from three donors.

Asterand's XpressWay^® Profiles are quantitative human gene expression data (216 data points), which are generated using quality controlled, qRT-PCR expressed mRNA with validated primer sets. Antibody candidates may be raised against a target with known expression in a tissue. TargetEvaluator^® allows alternative selection if a tissue with higher expression (e.g. pineal gland, seen in above stellar plot) at the mRNA level which may increase the likelihood of a successful antibody validation assay. This process will confirm the greatest imaging expression opportunity.

Step 2:
The antibody is then tested at 5 concentrations following 2 heat mediated antigen retrievals, in positive control tissues to find the most suitable IHC protocols for use with the antibody. A third option of proteolitic antigen retrieval is also available upon request.

Step 3:
Asterand provides the highest quality digital photomicrographs of the research antibody's immunoreactivity along with data regarding the optimum conditions for end user application of the antibody. This data increases validity for datasheets while expression images increase value for marketing efforts when uploaded to the company website for customer review.

Confirmation of optimal IHC detection conditions provides opportunity to select the best expression images for marketing purposes.

Benefits from Validating and Characterizing Research Antibodies
Asterand's service will increase the marketability and value of research antibodies through:

• High-quality digital photomicrographs and optimal dilution data for website and marketing materials
• Extended support and access to study samples via archiving
• Confidence in the value and marketability of research antibodies in human tissue by the Leader in human tissue-based research

Asterand provides real marketable solutions for your antibodies. Contact Asterand today to find out more about adding marketable value to your antibodies.advantage@asterand.com.